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1kb DNA-LADDER

ORDERING INFORMATION

Catalog #

Product

Amount

Price (EUR)

#
1kb DNA-Ladder
    50 g/ 500 l

#
1kb DNA-Ladder
5 x 50 g/ 500 l

DESCRIPTION

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10000 base pairs. The 1kb and 3kb bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

SOURCE

PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.

RANGE/BANDS

  • 250 - 10000 bp
  • 13 bands

USAGE INFORMATION

  • Load 5 l / lane
  • Containing bromophenol blue as tracking dyes

BASE PAIR ESTIMATION

Migration patterns of 1kb DNA Ladder in a 1% TAE agarose gel is listed below:


STORAGE

Store at 25C for 6 months.

Store at -20C for 24 months

For research use only. Caution: Not intended for human or animal diagnostic or therapeutic uses.